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KMID : 0357220070190040001
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Journal of Korean Society Physical Therapy
2007 Volume.19 No. 4 p.1 ~ p.14
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Relationship of the Signal Transduction-mediated Proteins and Enzymes to Contractility and Plasticity in Skeletal Muscles
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Kim Jung-Hwan
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Abstract
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Background: It is generally accepted that skeletal muscle contraction is triggered by nerve impulse and intracellular released from intracellular stores such as sarcoplasmic reticulum (SR). Specifically, this process, called excitation-contraction (E-C) coupling, takes place at intracellular junctions between the plasma membrane, the transverse (T) tubule L-type channel (dihydropyridine-sensitive L-rype channel, DHPR, also called tetrads), and the SR release channel (ryanodine-sensitive release channel, RyR, also called feet) of internal stores in skeletal muscle cells. Furthermore, it has been reported that the dependent and -independent contraction determine the expression of skeletal muscle genes, thus providing a mechanism for tightly coupling the extent of muscle contraction to regulation of muscle plasticity-related excitation-transcription (E-T) coupling.
Purpose: Expression and activity of plasticity-associated enzymes in gastrocnemius muscle strips have not been well studied, however.
Methods: Therefore, in this study the expression and phosphorylation of E-C and E-T coupling-related mediators such as protein kinases, ROS(reactive oxygen species)- and apoptosis-related substances, and others in gastrocnemius muscles from rats was examined.
Results: I found that expression and activity of MAPKs (mitogen-activated protein kinases, ERK1/2, p38MAPK, and SAPK/JNK), apoptotic proteins (cleaved caspase-3, cytochrome c, Ref-1, Bad), small GTP-binding proteins (RhoA and Cdc42), actin-binding protein (cofilin), PKC (protein kinase C) and channel (transient receptor potential channel 6, TRPC6) was observed in rat gastrocnemius muscle strips.
Conclusion: These results suggest that MAPKs, ROS- and apoptosis-related enzymes, cytoskeleton-regulated proteins, and channel may in part functionally import in E-C and E-T coupling from rat skeletal muscles.
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KEYWORD
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Excitation-contraction coupling, Excitation-transcription coupling, Signal transduction
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